Revolutionizing Live-Cell Microscopy: Advancements in Label-Free Imaging Techniques

In the realm of live-cell microscopy, fluorescence techniques have long dominated, yet challenges persist due to bleaching and motion blur caused by extended integration times. However, a breakthrough has emerged with Rotating Coherent Scattering (ROCS) microscopy, enabling high-contrast, label-free imaging of live cells with unprecedented speed and resolution.

Researchers at Freiburg University, Germany, have harnessed the power of our Cobolt 06-01 and Cobolt 05-01 laser series to study cell samples in minute details. ROCS microscopy capitalizes on intensity speckle patterns from all azimuthal illumination directions, aggregating thousands of acquisitions within a mere 10 milliseconds at a remarkable resolution of 160 nm and a rapid capture rate of 100 Hz.

Through sophisticated analysis methods, this research not only reveals how motion blur obscures cellular structures but also elucidates how slow structural motions can mask critical fast motions, offering profound insights into the dynamic processes within living cells.

The study heralds a new era in live-cell microscopy, promising unparalleled discoveries in cellular dynamics.